Expression and secretion of thaumatin from Aspergillus oryzae.
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چکیده
منابع مشابه
Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.
Two different strains, Aspergillus awamori TGDTh-4 and A. awamori TGP-3 overexpressing a synthetic gene encoding the plant sweet protein thaumatin, showed an unfolded protein response. To facilitate protein secretion, the chaperone BiPA gene was expressed in A. awamori under control of the strong constitutive promoter of the gpdA gene. A good correlation was observed between the level of the bi...
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Although the production of proteolytic enzymes by different strains of Aspergillus oryzae has been investigated in submerged culture (5, 15), in static liquid culture (3, 11), and on solid substratum (8-10), there appear to be few reports pertaining to the comparative evaluation of these different methods on the elaboration of proteinases by specific strains of A. oryzae. K. Mogi (13) compared ...
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A novel serine carboxypeptidase (EC 3.4.16.1) was found in an Aspergillus oryzae fermentation broth and was purified to homogeneity. This enzyme has a molecular weight of ca. 67,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity is 21 U/mg for carbobenzoxy (Z)-Ala-Glu at pH 4.5 and 25 degrees C. It has a ratio of bimolecular constants for ...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.2513